Solutions for Chick Embryology 10X PBS stock NaCl 175.3g KH2PO4 7.7g K2HPO4 (anhydrous) 25 g dH20 to 2L 10X Tyrode's saline NaCl 80 g KCl 2 g CaCl2.2H2O 2.71 g Na PO4.2H2O 0.5 g MgCl2.6H2O 2 g glucose 10 g dH2O to 1 liter, autoclave 4% PFA in PBS Heat 100 ml 1X PBS to 60ºC Add 4 g paraformaldehyde with stirring Add 0.5 ml 1 N NaOH and stir until clear Neutralize with 0.5 ml 1N HCl   ©Cebra-Thomas, 2000 Last Modified: 25 May 2005 [Lab Protocols | Students | Cebra-Thomas | Course | Links ]
			Ca+2/Mg+2 free Tyrode's solution NaCl 8.0 g KCl 0.3 g NaH2PO4.H2O 0.05 g dH2O to 1 liter
			Howard Ringer's solution NaCl 7.2 g CaCl2(anhyd.) 0.17g or CaCl2.2H2O 0.23g KCl 0.37g dH2O to 1 liter