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In
vitroculture of
chick heart
1.
Use sterile technique. Prewarm Howard's Ringers in petri
dish and culture media (2% FCS in DMEM) in organ culture
dish
to 37ºC on slide
warmer.
2.
Remove 3-day chick from egg; transfer to warm Howard's
Ringers. Dissect away extra-embryonic membranes.
3.
Using fine forceps, sever the trunk above and below the
heart region. Then, carefully remove the dorsal region of
the
embryo, leaving the heart
intact.
4.
Identify the sinus venosus, artium and ventricle. Measure
the heart beat, and determine the direction.
5.
Transfer the heart to warm culture media. Check for a change
in heart beat rate or pattern.
6.
Incubate in CO2 incubator overnight. Check for beat and
evidence of development the next day, and
thereafter.
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