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2. Add 100 ul AP substrate to
tubes. Check for staining after 10 minutes by transfering to
depression slide (no cover slip) and observing on 4X. To
stop the reaction, return to tube and add 0.5 ml
PBS.
3.
Allow embryos to settle for 10 minutes. Remove buffer to
about 100 ml and observe using depression slide.
Note:
Use wide-bore pipet tips (Cell-saver).
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