In vitroculture of early chick embryos 1. Use sterile technique. Prewarm Howard's Ringers in petri dish and agar/albumin culture dish to 37ºC on slide warmer. 2. Crack 1-day egg into large sterile petri dish. Place filter paper ring around blastodisk, and cut around it. Transfer filter paper ring with embryo to warm Howard's Ringers. Stage embryo. Gently wash away adherent yolk. 3. Transfer to agar/albumin dish filter paper-side down (ventral side of embryo is up). 4. Incubate in 37º incubator overnight. Check for evidence of development the next day, and thereafter.