Amphibians - Advanced Protocols Models of Amphibian gastrulation Organizer grafts Transplantation of eye-forming region- 1, 2 Transplantation of gill-forming region Neural crest transplant - 1, 2 Bisection of the heart field in axolotl -1, 2 Fate mapping in axolotl Inhibition of SHH signalling in amphibian embryos by treatment with cyclopamine -Xenopus, axolotl Lithium chloride treatment