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		Prep List
		Warm water incubator set at 40.0°C Incubator set at 28.5°C Wide-bore pasture pipettes Fine forceps Glass Pyrex dishes for collection and incubation of embryos Zebrafish embryo medium (NaH2PO4 0.01M 10mL; Na2HPO4 0.01M 10mL; Sodium citrate 0.1M 20mL; CaCl2 0.1M 15mL; dH20 945 mL) 4% paraformaldehyde in PBS 1% paraformaldehyde in PBS znp-1 antibody PBS 0.2% saponin in PBS Goat anti-mouse IgG+IgM-HRP (secondary antibody conjugated to peroxidase) @1/500 HRP substrate buffer HRP substrate (metal-enhanced DAB) 1:1 glycerol:CMFET 4:1 glycerol:CMFET Dissecting microscope Compound microscope Depression slides and coverslips