Results
The most concentrated dilution of FgF2, [4.0µl FgF2/10ml DMEM], demonstrated the lowest percentage of stained cells (Table 1). Whereas, the least concentrated dilution of FgF2, [0.25µl FgF2/10ml DMEM] demonstrated the highest percentage of stained cells, even higher than the control (Table 1). Generally, the stained cells in each culture were larger in size and often irregular in shape (Figure 2, A. & C.). Most myosin free cells were found in bundles (Figure 1, B. & C.). Whereas, the stained heavy chain myosin cells were isolated.

Table 1. Stage 14 segmental plates cells were plated on 1% gelatin. Three days after plating, cells were labeled with MF20 monoclonal antibody against myosin heavy chain. Percentage muscle (stained cells-unstained cells / total cells X 100) was determined after scoring cells. Values are the mean + standard deviation. The number of cultures scored was 3 for each condition. The concentrations of FgF2 were four-fold dilutions in 10 mls of culture medium (DMEM).

Control

FgF2 (0.25µl/10ml)

22.4 +5.7

FgF2
(1.0µl/10ml)

9.0 +5.11

91.0 +5.11

FgF2
(4.0µl/10ml)

6.2 +4.15

93.8 +4.15

% cells stained19.4 +2.04

% cells unstained

80.6 +2.04

77.6 +5.77