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		RESULTS Although many embryos died, some chicks lived in shell-less culture for 16 days. Cultured chicks were smaller than comparably aged chicks incubated in the shell. Chick embryos cultured for 16 days. Reference: Tuan, R.S., and M.H. Lynch (1983). Effect of experimentally induced calcium deficiency on the developmental expression of collagen types in chick embryonic skeleton. Dev. Biol. 100:374-386.