Effect of ethanol on zebrafish development

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Procedure:

1. Select embryos that are at the dome/30% epiboly stage stage (Figure 1).

2. Prepare three separate petri dishes that contain normal zebrafish embryo medium, a 2.5% ethanol zebrafish embryo medium solution, and a 1% ethanol embryo solution.

3. Place at least 10 of the selected embryos into each of the petri dishes and let them sit for 3 hours.

4. After 3 hours has passed, place all 3 sets of embryos into petri dishes with normal zebrafish embryo solution.

5. 24 hours later observe embryos for abnormalities and photograph them.

Figure 1: Zebrafish embryo in epiboly stage.

© Cebra-Thomas, 2001
Last Modified: 2 August 2001

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				Procedure: 1. Select embryos that are at the dome/30% epiboly stage stage (Figure 1). 2. Prepare three separate petri dishes that contain normal zebrafish embryo medium, a 2.5% ethanol zebrafish embryo medium solution, and a 1% ethanol embryo solution. 3. Place at least 10 of the selected embryos into each of the petri dishes and let them sit for 3 hours. 4. After 3 hours has passed, place all 3 sets of embryos into petri dishes with normal zebrafish embryo solution. 5. 24 hours later observe embryos for abnormalities and photograph them. Figure 1: Zebrafish embryo in epiboly stage.





		© Cebra-Thomas, 2001 Last Modified: 2 August 2001 [Lab Protocols \| Students \| Cebra-Thomas \| Course \| Links ]