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				Results & Conclusions: The zebrafish in the control group developed normally as was expected. At the stage that they were observed, they showed normal eye development. (figure 2). The embryos in the 1% ethanol solution also showed normal development in the region of the head (figure 3). Apparenty this concentration of ethanol was not sufficient to cause cyclopia. The 2.5% ethanol experimental group showed a limited number of deformed embryos. Out of the ten original embryos two failed to survive, though this was consistent with the survival rate of the control group. Out of the eight that survived, six developed normally and two were slightly deformed. It appears that under these experimental conditions a higher concentration of ethanol was needed to produce a higher frequency of teratogenic results. It was difficult to determine whether the two deformed emrbyos had full blown cyclopia but they did not have visible eyes (figure 4). Figure 2: An embryo from the control group, eye pigment can be clearly seen. Figure 3: Embryo from the 1% ethanol group showing normal eye development. Figure 4: Embryo from 2.5% ethanol group showing underdeveloped or absent eyes.
		© Cebra-Thomas, 2001 Last Modified: 2 August 2001 [Lab Protocols | Students | Cebra-Thomas | Course | Links ]