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The
study of acidic pH levels effecting the development of
zebrafish. This is being done to determine if acid rain
has any major effect on
developing embryos. Rainfall (pH 5.6) has a more acidic
value than that of normal water (pH 7
or neutral).
The Embryo medium, which is
used for developing dechorionated embryos, as well as the
other Hank's standard
solutions can be seen here.
Adding less NaOH buffer so that it will be less than a pH
7.2 level will change the
embryo medium. This is supposedly only pertinent if the
chorion has been removed. Hopefully the chorion will not
hinder pH interactions. The
slow addition of NaOH will enable for a fair range of acidic
to optimal pH reading levels. By
taking pictures of the embryos
prior to exposure to various pH solutions and comparing
these to pictures of the same
embryos further along development it will hopefully show
that pH level has en effect on the development of the
embryos.
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