1 2 3 4 5

Procedure
1) Zebrafish were marbled the previous night before collection of embryos
-The marbles in the tank bottom promote spawning when the next dawn comes
2) Embryos were selected for two varying cell stages of development
-first set were between the 2-4 cell stage of development
-second set was of embryos between 64-128 cells in development
3) pH levels used were: 7.2, 6.6, 6, 5, and 4.
-pH 7.2 is the control experiment

Note: Each of these pH levels was applied to each of the two cell stages previously mentioned. An image was taken of the initial embryos to compare with the developing embryos in the various pH solutions. Embryos were observed over a 48 hour period.

@ Cebra-Thomas, 2001

Last Modified: 2 August 2001


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