Procedure
1) Zebrafish were marbled
the previous night before collection of embryos
-The marbles in the tank
bottom promote spawning when the next dawn comes
2) Embryos were selected for
two varying cell stages of development
-first set were between the
2-4 cell stage of development
-second set was of embryos
between 64-128 cells in development
3) pH levels used were: 7.2,
6.6, 6, 5, and 4.
-pH 7.2 is the control
experiment
Note:
Each of these pH levels was applied to each of the two cell
stages previously mentioned. An image was taken of
the initial embryos to compare
with the developing embryos in the various pH solutions.
Embryos were observed over a
48 hour period.
|