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Procedure for UV irradiation

1) We put 15 embryos, randomly oriented in their chorion in 3 different petri dishes.
To produce the UV irradiation we used a 254 nm CL-1000 UV crosslinker.
To change the UV doses we can change the exposure times. Nevertheless, in this
experimentwe increased the irradiation without change the time of exposure.

2.) Exposure I ( 1.8 mJ/cm2)
We placed 15 embryos in a petri dish and then we put the petri dishes in the CL-1000 UV
crosslinker as explained previously.

3)Exposure II ( 3.6 mJ/cm2)
Same thing but the level of irradiation was doubled.

4)We also put 15 embryos in a petri dish and did not irradiated them. Theyserved
as reference during the observation (the next day).

5)We placed the 3 petri dishes to incubate at 28ºC in embryo media.

© Cebra-Thomas, 2000

Last Modified: 2 August 2001


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