Procedure for UV
irradiation
1)
We put 15 embryos, randomly oriented in their chorion in 3
different petri dishes.
To produce the UV
irradiation we used a 254 nm CL-1000 UV crosslinker.
To change the UV doses we
can change the exposure times. Nevertheless, in this
experimentwe
increased the irradiation without change the time of
exposure.
2.)
Exposure I ( 1.8 mJ/cm2)
We placed 15 embryos in
a petri dish and then we put the petri dishes in the CL-1000
UV
crosslinker as explained
previously.
3)Exposure
II ( 3.6 mJ/cm2)
Same thing but the level
of irradiation was doubled.
4)We
also put 15 embryos in a petri dish and did not irradiated
them. Theyserved
as reference during the
observation (the next day).
5)We
placed the 3 petri dishes to incubate at 28ºC in embryo
media.
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