Effect of UV on zebrafish development

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Results
In the early development, the rapid cleavage divisions are not affected by the UV-light. Anomalies start when epiboly starts. Treated embryos progress slower than untreated embryo. So, the fundamental components driving the epiboly should start to work in the late zygote. The experiments gave rise to three level of impair:

Type A-->Slightly affected.
The blastoderm has covered the yolk cell up to 80% and shows a well-formed anterior axis
rudiment onthe dorsal side.

Type C-->The more affected. Type B-->Affected.
Retardation of epiboly but do not formThe blastoderm rounded up forming a vesicle on an axis rudiment. top of the yolk mass.

All the control gave rise to normal embryo with normal epiboly movement. As shown on the table, the importance of the "mutation" are proportional with the level of UV exposure.

The irradiated embryos are disorganizedand less microtubules were found in the cytoplasmic layer of the yolk sphere (Strahle). Thus, since epiboly is the only one movement in the embryo which is affected, it should be driven by motors that use microtubules.

(Results are from Uwe Strahle and Suresh Jesuthasan, Development 119, 909-919 (1993) )

Last Modified: 2 August 2001

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		Results In the early development, the rapid cleavage divisions are not affected by the UV-light. Anomalies start when epiboly starts. Treated embryos progress slower than untreated embryo. So, the fundamental components driving the epiboly should start to work in the late zygote. The experiments gave rise to three level of impair:
				Type A-->Slightly affected. The blastoderm has covered the yolk cell up to 80% and shows a well-formed anterior axis rudiment onthe dorsal side.
		Type C-->The more affected. Type B-->Affected. Retardation of epiboly but do not formThe blastoderm rounded up forming a vesicle on an axis rudiment. top of the yolk mass. All the control gave rise to normal embryo with normal epiboly movement. As shown on the table, the importance of the "mutation" are proportional with the level of UV exposure. The irradiated embryos are disorganizedand less microtubules were found in the cytoplasmic layer of the yolk sphere (Strahle). Thus, since epiboly is the only one movement in the embryo which is affected, it should be driven by motors that use microtubules. (Results are from Uwe Strahle and Suresh Jesuthasan, Development 119, 909-919 (1993) )