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		7. Transfer the excised piece of tissue to the slit in the host. Gently, yet firmly, push the whole mass of cells through the narrow incision, it may be necessary to enlarge the slit to accommodate the transplant, but a tight-fitting transplant is desirable. 8. Once the transplant has been pushed into the slit, quickly cover it with the flat surface of a thin coverslip. The weight of the cover slip should hold the tissue in place. 9. After and hour, the piece of coverslip should be carefully removed. If the transplant has not healed, place it again under pressure, with a piece of coverslip, for an additional half hour. After healing is complete, transfer the embryo to spring water or ringers solution. 10. Observe to see if the transplanted tissue forms a second neural fold.