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		Prep list for RNA isolation, RNA gel and cDNA synthesis Solutions
		10X MOPS buffer:		To 400 ml DEPC-treated dH2O, add 20.9 g MOPS 8.3 ml 3 M NaAcetate 10 ml 0.5 M EDTA, pH 8 pH to 7.0 with 10N NaOH or acetic acid Cover with aluminum foil and autoclave
		Howard Ringer's solution 7.2 g NaCl 0.17g CaCl2(anhyd.) or 0.23g CaCl2.2H2O 0.37g KCl add dH2O to 1 liter 5X TBE buffer 54 g Tris base 27.5 g boric acid 20 ml 0.5 M EDTA, pH 8 dH2O to 1 liter Equipment Polytron homogenizer Safety goggles Lab coats Variable speed microfuge P1000, P200 and P20 pipetmen UV spectrophotometer and cuvette Water bath (41ºC) Shaking incubator (55ºC and 65ºC) Heating stirrer Power supply RNase-free minigel box Supplies Small, medium & large gloves Bench paper Petri dishes (sterile) Pipetmen tips (barrier, sterile & RNase-free) for P1000, P200 and P20 Microfuge tubes (sterile & RNase-free) 8 ml tubes (sterile) Agarose Polypropylene bags for chemical waste Storage box Tube poppers