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Solutions
DEPC (Sigma) to treat Howard's,
dH2O
70 % ethanol (to clean eggs,
etc.)
Howard's Ringer's (sterile, RNase-free)
TRIZOL (Sigma)
Chloroform (RNase-free)
Isopropanol (RNase-free)
75 % ethanol (RNase-free)
dH2O
(sterile, RNase-free) to make 1X MOPS, clean gel box,
homogenizer probe, etc. PRIME
RNase inhibitor (30 ul/400 ul
dH2O
for 10 samples) to resuspend RNA (5'3')
10X MOPS (powder from Sigma
made up in DEPC-dH2O)
1X MOPS
37% formaldehyde (Sigma)
RNA sample buffer (Sigma)
1 mg/ml EtBr
cDNA synthesis kit (AMV RT, Life Sciences)
For
PCR reaction
PCR tubes
PCR reaction mix
dH2O
mineral oil
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