1 2

Solutions
DEPC (Sigma) to treat Howard's, dH
2O
70 % ethanol (to clean eggs, etc.)
Howard's Ringer's (sterile, RNase-free)
TRIZOL (Sigma)
Chloroform (RNase-free)
Isopropanol (RNase-free)
75 % ethanol (RNase-free)
dH
2O (sterile, RNase-free) to make 1X MOPS, clean gel box, homogenizer probe, etc. PRIME RNase inhibitor (30 ul/400 ul dH2O for 10 samples) to resuspend RNA (5'3') 10X MOPS (powder from Sigma made up in DEPC-dH2O)
1X MOPS
37% formaldehyde (Sigma)
RNA sample buffer (Sigma)
1 mg/ml EtBr
cDNA synthesis kit (AMV RT, Life Sciences)

For PCR reaction
PCR tubes
PCR reaction mix
dH
2O
mineral oil

@2004 Cebra-Thomas

Last Modified: 17 August 2004


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