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Discussion
The
purpose of our research was to explore the effect of lead on
neural crest cells. We hypothesized that introducing lead to
post-migratory NC cells would result in a higher rate of
spontaneous neurite outgrowth than normal. It is hard with
such a small sample group to draw conclusions. However, we
did see an increase in the number of branches coming off of
neurons that were exposed to the lead acetate.
Looking at the results of
our experiment, a few flaws in our experimental design
become apparent. The number of conditions and explants that
we cultured was too small. The next time this experiment is
run it would be beneficial to increase the number of tubes
cultured. During our experiment, we only explored the
effects of two, very disparate, concentrations of
Pb-Acetate. Inthe next trial,
the cultured NC cells should be exposed to a wider range of
Pb-Acetate concentrations. With more trials, better explants
will be used to culture the NC cells. We also believe that
unfamiliarity with the staining procedure might have
effected our results.
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