Figures

Concentration	# Embryos Alive	# Embryos Dead
Control (ZES)	16	2
0.025 M	21	8
0.05 M	16	13
0.1 M	16	17
0.2 M	0	24

Table 1. The number of surviving and dead embryos after a 20 hour treatment in different concentrations of valproic acid.

Figure 3. F6 antibody stain for somites in an embryo treated with 0.05 M valproic acid solution. Somite fusion of adjacent pairs and mis-segmentation of somites are characteristic of Class I anomalies.

Figure 5. Zebrafish embryo treated with 0.2M valproic acid solution that failed to develop.

Figure 2. F6 antibody stain for somites in control embryo demonstrating normal somite development at 28 hours after fertilization.

Figure 4. F6 antibody stain for somites in an embryo treated with 0.1 M valproic acid solution. Disorganized and scrambled somites are characteristic of Class II anomalies.