1 2 3 4

In the second part of the experiment the potential effects of ethanol were examined by keeping a constant amount of ethanol in each sample. Using the data obtained from the first part of the experiment, we knew that at cyclopamine treatments above 100 micromoles there were no surviving embryos making these concentrations useless. The following concentrations (in micromoles) were used, resulting in a certain number of mutations, deaths, and normal growths. spacer Control 50 cyclopamine 50 ethanol control 100 cyclopamine 100 ethanol control Normal 15 6 w/ minor changes 7 w/minor changes 0 0 Mutated 0 3 major 3 major 8 0 Dead 0 6 5 7 15