Figure 3: Culture 1; Skin originally from 8 day chicks at day 4 in culture (day 12 for the chick) showing short buds. Skin cultured in Hank’s Balanced Salt Solution 1X, on Transwell 3452 culture inserts (24mm diameter), placed in 5% CO2 incubator at 37¾C. (A) Note various stages of short buds as we move from the dorsal area (D) to the dorsolateral (DL). Development of the spinal tract (ST) is apparent. Note also islands of blood development. (B) Close-up of developing islands of blood amongst the early feather buds (short bud
stage). (C) Line drawing diagram of dorsal view of chick body. Red arrow highlights location of spinal tract.