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Figure 5: Culture 1; Skin originally from 8 day chicks at day 15 in culture
(day 22 for the chick) showing late stage long buds. Skin cultured in Hank’s
Balanced Salt Solution 1X, on Transwell 3452 culture inserts (24mm diameter),
placed in 5% CO2 incubator at 37¾C. (A) Note that feather buds in the center
seem to be more closely grouped together, as well as somewhat more advanced.
(B) Close-up of late long feather buds, highlighting the dermal papilla,
papillary ectoderm, epidermal collar, and presumptive feather filament.
Note the pigmentation of the presumptive feather filament and dermal papilla.
(C) Line drawing diagram of B, further detailing feather bud anatomy. |
