Feather Bud Development |
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Figure 6: Culture 1; Skin originally from 8 day chicks at day 4 in culture (day 12 for the chick) showing irregularly forming feather buds. Skin cultured in Hank’s Balanced Salt Solution 1X, on Transwell 3452 culture inserts (24mm diameter), placed in 5% CO2 incubator at 37¾C. (A) Note variance in shapes of feather buds as well as lack of any discernable pterylae. (B) Close-up of culture, detailing irregularities in dermal papillae. | ||