1 2
		Solutions for Sea Urchin ASW: Artificial sea water (MBL) NaCl 24.72 g KCl 0.67 g CaCl2.2H2O 1.36 g MgCl2.6H2O 4.66 g MgSO4.7H2O 6.29 g NaHCO3 0.18 g dH2O to 1 liter Sulfate-free Sea water NaCl 27.72 g KCl 0.67 g CaCl2.2H2O 1.36 g MgCl2 9.32 g NaHCO3 0.18 g dH2O to 1 liter		Development
				0.5 M KCl to induce gamete shedding 3.73 g KCl/ 100 ml dH2O LSSW: Low sodium sea water (50 mM) NaHCO3 0.2 g Choline Cl 28.3 g KCl 0.7 5g CaCl2.2H2O 1.6 g MgCl2.6H2O 5.5 g MgSO4.7H2O 7.1 g dH2O to 1 liter, adjust pH to 7.8 CaFSW: Calcium-free Seawater NaCl 25.6 g KCl 0.7 g MgSO4.7H2O 11.9 g NaHCO3 0.5 g dH2O to 1 liter