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		10X PBS stock NaCl 175.3g KH2PO4 7.7g K2HPO4 (anhydrous) 25 g dH20 to 2L 4 mg/ml soybean trypsin inhibitor 40 mg SBTI/10 ml Artificial sea water Transfer 1 ml aliquots to clean microfuge tubes and store frozen. Alkaline phosphatase substrate buffer (100 mM NaCl , 100 mM Tris, pH 9.5, 50 mM MgCl2 ) 5 M NaCl 2 ml 2 M Tris, pH 9.5 5 ml 4.9 M MgCl2 1 ml dH2O (ultrapure) to 100 ml © Cebra-Thomas, 2000 Last Modified: 25 May 2004 [Lab Protocols | Students | Cebra-Thomas | Course | Links ]