1 2 3 4 5

		Results The 2 mL treatment included two wing discs (fig. 6, 7). By 2 hours, wing eversion is clearly visible (fig. 13, 14). After 24 hours, further eversion was observed (fig 19-21). The 5 mL treatment included a pair of leg discs (fig. 8). After 2 hours of exposure, the tips of the discs appear to protrude further out than initially at 0 hours (fig. 15). After 24 hours of exposure, additional eversion of the legs has taken place (fig. 22). The 8 mL treatment included a wing, eye, and leg disc (fig. 9-11). The leg disc has everted noticeably by 2 hours (fig. 16), while the eye disc did not change in appearance (fig. 17) (an image of the wing disc at 2 hours was not obtained due to technician oversight). However after 24 hours, the wing, eye, and leg discs have everted dramatically (fig. 23-25). The control treatment (ethanol) discs showed no dramatic change in appearance during the 24-hour period as expected (fig 12, 18, 26).   Figure 6. Wing imaginal disc in 2 uL ecdysone at 0 hr. Figure 7. Wing imaginal disc in 2 uL ecdysone at 0 hr. Figure 8. Leg imaginal discs in 5 uL ecdysone at 0 hr. Figure 9. Wing imaginal disc in 8 uL ecdysone at 0 hr. Figure 10. Eye imaginal disc in 8 uL ecdysone at 0 hr. Figure 11. Leg imaginal disc in 8 uL ecdysone at 0 hr. Figure 12. Haltere imaginal discs in ethanol at 0 hr. Figure 13. Wing imaginal disc in 2 uL ecdysone at 2 hr. Figure 14. Wing imaginal disc in 2 uL ecdysone at 2 hr. Figure 15. Leg imaginal discs in 5 uL ecdysone at 2 hr. Figure 16. Leg imaginal disc in 8 uL ecdysone at 2 hr. Figure 17. Eye imaginal disc in 8 uL ecdysone at 2 hr. Figure 18. Haltere imaginal discs in ethanol at 2 hr. Figure 19. Wing imaginal disc in 2 uL ecdysone at 24 hr. Figure 20. Wing imaginal disc in 2 uL ecdysone at 24 hr. Figure 21. Wing imaginal disc in 2 uL ecdysone at 24 hr. Figure 22. Leg imaginal discs in 5 uL ecdysone at 24 hr. Figure 23. Wing imaginal disc in 8 uL ecdysone at 24 hr. Figure 24. Eye imaginal disc in 8 uL ecdysone at 24 hr. Figure 25. Leg imaginal disc in 8 uL ecdysone at 24 hr. Figure 26. Leg imaginal discs in ethanol at 24 hr.