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		Results In the control group embryos, anterior-posterior patterning was normal, with full formation of the terminus structures, the retina, and the lens of the eye (Figure 1A). In embryos treated with 0.15 M LiCl, the eyes appear to have developed normally, but the eye shown in Figure 1B is relatively closer to the anterior terminus than the eye in Figure 1A. The 0.15 M LiCl-treated embryos exibited slightly stunted growth at their anterior and posterior termini; the tails were also slightly bent and rounded at the tip (Figure 2B. The 0.30 M LiCl-treated embryos did not develop eyes, and exhibited slight curvature of the dorsal region (Figure 1C). These embryos exhibited even less development of the anterior and posterior termini than the 0.15 M embryos, with stubby tails and large yolk sacs (Figure 2C). All embryos in Figure1 exhibit development of ears, liver, and the CNS. The average survival rate for embryos in the 0.00 M, 0.15 M, and 0.30 M solutions were 19/19, 18/20, and 15/21 respectively. The 0.45 M LiCl -treated embryos had lower viability, with only seven of the original nineteen embryos surviving the 24-hour period from the time of incubation to the time of data collection. The resulting embryos were fragile, and some did not survive the dechorionation process and their cells became disaggregated by the pull of the forceps and the influx of Zebrafish embryo medium into the opened chorion. This group displayed a lack of anterior-posterior development, with no definable head structures, and stunted tail development (Figure 2D). A 0.00 M LiCl B 0.15 M LiCl C 0.30 M LiCl Figure 1 (A) Control embryo, typical anterior/posterior patterning with fully-formed anterior structures including the eye, forebrain, CNS, ear and liver (B) Embryo induced with 0.15 M LiCl shows liver, ear, eye, forebrain, and CNS development (C) 0.30 M LiCl-induced embryo shows no eye formation, though ear and liver are present.   A 0.00 M LiCl B 0.15 M LiCl C 0.30 M LiCl D 0.45 M LiCl Figure 2 Photographs taken 26 hours after LiCl induction (A) Control embryo shows normal tail formation (B) Embryo shows normal tail development (C) Tail appears stunted and crooked, gut is shortened, head is smaller, and yolk is enlarged and misshapen (D) Head and tail are stunted, anterior structures are missing, yolk is enlarged.
		J. N. White 5/13/04