Effect of lithium on fish development

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Acknowledgements

I would like to recognize several individuals for making this experiment possible. Thank you first of all to my lab group members, Kristi La Salle, Brandt Rakowski, Katy Lewis, and Erin Betters for isolating the sphere-stage Zebrafish embryos used in this experiment. I would like to recognize Peter Burgess of Franklin and Marshall College who conducted a similar experiment on Medaka embryos that inspired me to conduct this experiment. Thanks to the lab personnel responsible for feeding the fish, and to Professor Judy Cebra-Thomas and Fraser Tan for preparing the LiCl concentrations used in this experiment. Thanks to Scott Stachel and his colleagues for providing useful information on previous experiments in lithium induction of Zebrafish and to Scott Gilbert for providing a wonderful resource for being able to interpret the data collected in this experiment. Additionally, I would like to thank William Gresh for advising me in the writing of this lab report.

References

Gilbert, Scott. Developmental Biology, 2003. 7th edition. Sinauer Associates Inc. Massachusetts: p. 345-347, 353

"Fish Protocols". Developmental Biology Lab. 2003. <http://www.swarthmore.edu/NatSci/sgilber1/DB_lab/Fish/fish_protocols.html>.

Detrich, H. William and Monte Westerfield, ed. Methods in Cell Biology: Volume 59. 1999. Academic Press. San Diego: p. 164-166.

Stachel, Scott E., Grunwald, David G., Myers, Paul Z. 1993. "Lithium perturbation and goosecoid expression identify a dorsal specification pathway in the pregastrula zebrafish". Development 117: 1261-1274.

"Zebrafish Information Network: Stages of Embryonic Development of the Zebrafish, Fig. 8." Kimmel et. al. <http://zfin.org>.

Stewart, RM and JC Gerhart. 1990. "The anterior extent of dorsal development of the Xenopus embryonic axis depends on the quantity of organizer in the late blastula." Berkeley.

J. N. White 5/13/04

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		Acknowledgements I would like to recognize several individuals for making this experiment possible. Thank you first of all to my lab group members, Kristi La Salle, Brandt Rakowski, Katy Lewis, and Erin Betters for isolating the sphere-stage Zebrafish embryos used in this experiment. I would like to recognize Peter Burgess of Franklin and Marshall College who conducted a similar experiment on Medaka embryos that inspired me to conduct this experiment. Thanks to the lab personnel responsible for feeding the fish, and to Professor Judy Cebra-Thomas and Fraser Tan for preparing the LiCl concentrations used in this experiment. Thanks to Scott Stachel and his colleagues for providing useful information on previous experiments in lithium induction of Zebrafish and to Scott Gilbert for providing a wonderful resource for being able to interpret the data collected in this experiment. Additionally, I would like to thank William Gresh for advising me in the writing of this lab report. References Gilbert, Scott. Developmental Biology, 2003. 7th edition. Sinauer Associates Inc. Massachusetts: p. 345-347, 353 "Fish Protocols". Developmental Biology Lab. 2003. <http://www.swarthmore.edu/NatSci/sgilber1/DB_lab/Fish/fish_protocols.html>. Detrich, H. William and Monte Westerfield, ed. Methods in Cell Biology: Volume 59. 1999. Academic Press. San Diego: p. 164-166. Stachel, Scott E., Grunwald, David G., Myers, Paul Z. 1993. "Lithium perturbation and goosecoid expression identify a dorsal specification pathway in the pregastrula zebrafish". Development 117: 1261-1274. "Zebrafish Information Network: Stages of Embryonic Development of the Zebrafish, Fig. 8." Kimmel et. al. <http://zfin.org>. Stewart, RM and JC Gerhart. 1990. "The anterior extent of dorsal development of the Xenopus embryonic axis depends on the quantity of organizer in the late blastula." Berkeley.
		J. N. White 5/13/04