|
|
Removal of the Eye Forming region:
Modified from Hamburger, V. 1960. A manual of
Experimental Biology. The University of
Chicago Press,
pp.107-109.
1)Position the donor embryo
so that the anterior part of the neural fold is crearly
visible.
2) Using eye-brow knives make very shallow cuts in the
anterior portion of the embryo.
3)After the cuts are made
carefully, not to tair the entire embryo apart, take off a
very thin layer of the
tissue.
|
|
|
|
Preparation of Albino Recipient Embryo:
1)Place
the albino embryos with the flank afrea facing up.
2)Make an incision starting
from the anterior to the posterior of the embryo.
3)Carefully transfer the
grafts to the petri dish containing the albino embryo.
4)Place the graft into the
flank cut of the albino. Make sure it stays in place so that
the
wound can heal.
5) Observe the embryos after a few days to see whether the
grafts have developed into an
eye. Take digital images of
the grafted embryos.
Use
clean technique; dip tools and pipettes in 70% EtOH and then
into sterile HBSt
beforeusing.
|
|