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 | Removal of the Eye Forming region:
 Modified from Hamburger, V. 1960. A manual of
         Experimental Biology. The University of
 Chicago Press,
         pp.107-109.
 
 1)Position the donor embryo
         so that the anterior part of the neural fold is crearly
         visible.
 2) Using eye-brow knives make very shallow cuts in the
         anterior portion of the embryo.
 3)After the cuts are made
         carefully, not to tair the entire embryo apart, take off a
         very thin layer of the
         tissue.
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 | Preparation of Albino Recipient Embryo:
 1)Place
         the albino embryos with the flank afrea facing up.2)Make an incision starting
         from the anterior to the posterior of the embryo.
 3)Carefully transfer the
         grafts to the petri dish containing the albino embryo.
 4)Place the graft into the
         flank cut of the albino. Make sure it stays in place so that
         the
 wound can heal.
 5) Observe the embryos after a few days to see whether the
         grafts have developed into an
 eye. Take digital images of
         the grafted embryos.
   Use
         clean technique; dip tools and pipettes in 70% EtOH and then
         into sterile HBSt 
         beforeusing. 
 
 
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