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Materials and Methods:
We will use 36 2-day old
chicken embryos at H&H stage 12 for this experiment. We
will place twelve eggs into three groups: control, 12.5ug
and 25ug of lead acetate. The control group will receive
distilled water rather than lead acetate. We will inject
0.1ml of the different concentrations of lead acetate or
distilled water into the yolk sac of the 2-day old embryos
with a needle and seal the hole with scotch tape. We will
incubate the embryos in 37C for two days. After incubation,
the chick embryos will be four days old and should be
H&H 24. We will remove all 12 embryos from each group
(control, 12.5ug, and 25ug) from the shell and yolk sac and
observe the development under a dissecting microscope. We
will stage the embryos and observe any morphologic
malformations of the neural components of the chick embryos,
such as the fore-, mid-, and hind-brain.
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