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		Experimental Design 1st Experiment 30 eggs were collected and incubated for 3 days. 24 of the eggs were used as the nicotine treatment group and the other 6 were used as controls. The nicotine solution applied consisted of 1.0 mg of nicotine and 0.1 mL of Howard's ringers solution. To administer the nicotine solution, a small piece of the shell was removed and the nicotine was added directly on top of the embryo. The egg was taped up and placed back into the 37oincubator. The controls were treated the same as the treatment group, except they received 0.11 mL of Howard's ringers solution instead of nicotine. Eggs were treated once and monitored for 3 days. 2nd Experiment 30 eggs were collected and incubated for 3 days. 12 of the eggs were administered 0.5 mg of nicotine in 0.1 mL of Howard's ringers solution. The other 12 eggs were administered with 0.25 mg of nicotine and 0.1 mL of Howard's ringers solution. The 6 controls were treated the same as the nicotine treatment group, except they were administered 0.11 mL of Howard's ringers solution. The nicotine solution was injected directly into the yolk of the egg using a 1cc syringe. Eggs were taped up and placed in a 37o incubator. Eggs were treated every 3 days. Following the first treatment, eggs were monitored everyday for viability. Pictures were taken using an Olympus DP10 microscope and digital camera.