|
|
Results
First
Experiment
After treatment with 1.0
mg of nicotine, all 24 chick embryos were dead. The embryos
were removed, but no development could be determined. The 6
control embryos were still alive.
Second
Experiment
After approximately 24
hours following treatment, 2 of the embryos from the 0.5 mg
treatment group were dead. By 48 hours 6 more embryos from
the0.5 mg group were dead and
9 embryos from the 0.25 mg group were dead. At 72 hours
after treatment, two more embryos, one from each group, had
died. When examined, the two embryos revealed developmental
retardation in the cranial region, limbs, and eyes
(Image
1). 3 days following the
second treatment 3 more embryos from the 0.5 mg group were
dead. At this point, all embryos from the 0.5 mg group were
dead and 2, 0.25 mg nicotine treated embryos remained. On
day 6 following the second treatment both 0.25 mg treated
embryos had died. All controls were still viable. Treatment
continued until day 13 when the 2 remaining embryos were
removed and examined (Image
2). To prevent further
contamination, the experiment was stopped 5 days short
because of the presence of mold in 2 of the controls. One
embryo (egg #8) exhibited low body length and birth weight
when compared to the control group (Image
3). The second embryo (egg
#9) displayed an apparent redness of the skin. Veins
appeared to protrude from the surface of the body.
(Image
4). All controls exhibited
normal development (Image
5).
|
|