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		Protocol 1) Remove 2-3 day chick embryos using the ring explant technique and store them in sterile Howard's balanced salt solution. 2) Dilute 20µl of Lipofectin® Reagent (Life Technologies, catalog no. 18292-011) into 200µl OptiMem® serum-free medium (Life Technologies) and allow it to stand at room temperature fo 30-45 minutes (Sol. A) 3) For each transfection, dilute 50 µg GFP DNA in 100µl of OptiMem® (Life Technologies) serum-free medium in a sterile microfuge tube (Solution B). 4) Combine Solutions A and B, stir gently and incubate at room temperature for 10-15 minutes to make Solution C. 6) Wash embryos with 2 ml serum free medium. 7) Add 1 ml OptiMem® serum-free medium to the dish containing Solution C. Mix gently and pour over embryo. 8) Incubate the cells for 8 hours in a 5% CO2 incubator at 37ºC. 9) Transfer embryos to albumin agar plates and incubate for 48-72 hours more in a 37º C incubator at 37ºC. 10) Observe embryos using fluorescent microscope 48-72 hours after transfection.