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Materials Required
Mature zebrafish on 10 hr/14
hr-dark/light cycle
Valproic acid
Zebrafish embryo
medium
PBS
0.1% BSA in PBS
0.2% saponin in PBS
F6 primary mouse antibody (stain for somite boundaries)
Secondary goat anti-mouse conjugated HRP antibody
HRP substrate buffer
HRP substrate (metal-enhanced DAB) [Pierce
Chemical]
1:1 glycerol:CMFET
4:1 glycerol:CMFET
Alcian Green Stain
5% Tri-chloroacetic acid (TCA)
70% Ethanol
soap-free 60 mm glass Petri dishes
incubator @28 degrees celsius
© Cebra-Thomas, 2001
Last Modified: 14 July 2014
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