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Background:The
goal of this experiment is to determine if the gill organ
field is specified at the particular time that it is
transplanted. According to Gilbert (2000), a cell or a
tissue is said to be specified when it is capable of
differentiating as it would have when placed in a neutral
environment such as a petri dish or test tube or after
transplantation to an ectopic site. An organ field is a
large region of the embryo in which cells are specified
generally (leg, gill) but are not specified for a specific
organ such as a toe or finger. Organ fields are developed in
the axolotl through induction. An interaction at close range
between two or more cells or tissues that have different
properties is called induction (Gilbert, 2000). There are
two components necessary for inductive interaction. The
first component is the inducer, which is the tissue or cells
that produce a signal(s) that will change the cellular
behavior or activity of the other cells or tissue. The
second component is the responder, which is the tissue or
group of cells that are being induced (Gilbert, 2000).
Coordination in the construction of organ fields is achieved
as one group of cells changes the behavior of an adjacent
set of cells, which will cause the cells to change shape,
mitotic rate, or fate (Gilbert, 2000). Once the organ field
is specified, inductive effects will help further develop
the field into the final organ. By transplanting the gill
organ field from a wild type axolotl into an albino axolotl
and vice versa, we plan on examining if the gill develops as
it would have in its original environment or if it is
influenced by the surrounding tissues. We expect the gill to
develop as it would have in its original
environment.
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