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5. Once the surgery is complete, be
sure to take pictures of a normal donor and recipient and
then the newly transplanted graft of the recipient. Pictures
should be taken at various stages to observe development (to
be determined at discretion of researcher).
6. The embryos should be left in HBSt at room temperature to
continue to development. Observe both donors and recipients
as time continues to examine the effect of the transplanted
graft.
7. Make OBSERVATIONS as development continues...this is the
key to obtaining results!
8. After the first day, it is important to replace the
operating solution with 10% modified Steinberg's solution.
The graft will develop better in a lower concentration of
certain chemicals. To do this, simply pipette off the
operating solution and pour in the 10% modified Steinberg's
solution.
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